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blockpro tm blocking buffer  (Energenesis Biomedical)

 
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    Structured Review

    Energenesis Biomedical blockpro tm blocking buffer
    Blockpro Tm Blocking Buffer, supplied by Energenesis Biomedical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blockpro+tm+blocking+buffer/blocking+blockpro+buffer+tm/pmc13114095-95-13-17
    Average 86 stars, based on 1 article reviews
    blockpro tm blocking buffer - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Seroprevalence of Neospora caninum Infection in Shelter Dogs from Hanoi, Vietnam
    Article Snippet: .. Subsequently, 100 μL of canine serum samples diluted 1:100 or 1:200 in 1× BlockPro TM Blocking buffer (Energenesis Biomedical, Taipei, Taiwan) were added to each well and incubated for 1 h at room temperature. .. After washing five times with PBS-T, 100 μL of horseradish peroxidase goat anti-dog IgG (H + L) antibodies (Bio-Rad, Hercules, CA, USA), diluted 1:8000 in BlockPro TM buffer, were added to each well and incubated for 1 h at room temperature.

    Article Title: Notoginsenoside R1 Attenuates Cisplatin-Induced Ototoxicity by Inducing Heme Oxygenase-1 Expression and Suppressing Oxidative Stress
    Article Snippet: .. The cells were incubated with polyclonal primary antibodies against 4-HNE (1:100; Abcam, Cambridge, UK) in BlockPRO TM blocking buffer (Energenesis Biomedical Co., Ltd., Taipei, Taiwan) in a humidified chamber for 1 h and then stained with a secondary antibody (donkey anti-rabbit conjugated to Alexa Fluor 488, 1:500; Molecular Probes; Thermo Fisher Scientific, Waltham, MA, USA) for an additional 1 h. Then, the cells were mounted in DAPI Fluoromount-G ® mounting medium. .. Images were captured with a confocal laser scanning microscope (Zeiss LSM 880, Carl Zeiss, Jena, Germany).

    Article Title: Notoginsenoside R1 Attenuates Cisplatin-Induced Ototoxicity by Inducing Heme Oxygenase-1 Expression and Suppressing Oxidative Stress
    Article Snippet: Immunoblot images were acquired with a UVP ChemStudio Imager (Analytik Jena Co., Upland, CA, USA) using Immobilon ® Western Chemiluminescent HRP Substrate (ECL; Merck KGaA, Darmstadt, Germany). .. The cochlear explants were fixed with 4% paraformaldehyde for 30 min at RT and then incubated with the permeabilization solution for 30 min. After the explants were washed, they were blocked with BlockPRO TM blocking buffer (Energenesis Biomedical Co., Ltd., Taipei, Taiwan). .. The cochlear explants were incubated with anti-myosin VIIa (Santa Cruz Biotechnology, Dallas, TX, USA), anti-4-HNE (Abcam, Cambridge, UK) and anti-iNOS (Novus Biologicals, LLC, Centennial, CO, USA) antibodies for 2 h at RT.

    Article Title: Notoginsenoside R1 Attenuates Cisplatin-Induced Ototoxicity by Inducing Heme Oxygenase-1 Expression and Suppressing Oxidative Stress
    Article Snippet: .. Aliquots of cell lysate were separated on mPAGE TM 4–12% Bis-Tris polyacrylamide gels (Merck KGaA, Darmstadt, Germany), transferred to polyvinylidene difluoride membranes (Millipore, Billerica, MA, USA), blocked with BlockPRO TM blocking buffer (Energenesis Biomedical Co., Ltd., Taipei, Taiwan), probed with anti-cleaved Caspase-3 (Cell Signaling Technology, Danvers, MA, USA), anti-HO-1 (Cell Signaling Technology, Danvers, MA, USA), and anti-actin (Merck KGaA, Darmstadt, Germany) antibodies at 4 °C overnight, washed with PBST, and incubated with horseradish peroxidase-conjugated secondary anti-rabbit or anti-mouse antibodies (1:10,000; Cytiva, Marlborough, MA, USA) diluted with BlockPRO TM blocking buffer (Energenesis Biomedical Co., Ltd., Taipei, Taiwan) for 1 h at room temperature (RT). .. Immunoblot images were acquired with a UVP ChemStudio Imager (Analytik Jena Co., Upland, CA, USA) using Immobilon ® Western Chemiluminescent HRP Substrate (ECL; Merck KGaA, Darmstadt, Germany).

    Incubation:

    Article Title: Seroprevalence of Neospora caninum Infection in Shelter Dogs from Hanoi, Vietnam
    Article Snippet: .. Subsequently, 100 μL of canine serum samples diluted 1:100 or 1:200 in 1× BlockPro TM Blocking buffer (Energenesis Biomedical, Taipei, Taiwan) were added to each well and incubated for 1 h at room temperature. .. After washing five times with PBS-T, 100 μL of horseradish peroxidase goat anti-dog IgG (H + L) antibodies (Bio-Rad, Hercules, CA, USA), diluted 1:8000 in BlockPro TM buffer, were added to each well and incubated for 1 h at room temperature.

    Article Title: Notoginsenoside R1 Attenuates Cisplatin-Induced Ototoxicity by Inducing Heme Oxygenase-1 Expression and Suppressing Oxidative Stress
    Article Snippet: .. The cells were incubated with polyclonal primary antibodies against 4-HNE (1:100; Abcam, Cambridge, UK) in BlockPRO TM blocking buffer (Energenesis Biomedical Co., Ltd., Taipei, Taiwan) in a humidified chamber for 1 h and then stained with a secondary antibody (donkey anti-rabbit conjugated to Alexa Fluor 488, 1:500; Molecular Probes; Thermo Fisher Scientific, Waltham, MA, USA) for an additional 1 h. Then, the cells were mounted in DAPI Fluoromount-G ® mounting medium. .. Images were captured with a confocal laser scanning microscope (Zeiss LSM 880, Carl Zeiss, Jena, Germany).

    Article Title: Notoginsenoside R1 Attenuates Cisplatin-Induced Ototoxicity by Inducing Heme Oxygenase-1 Expression and Suppressing Oxidative Stress
    Article Snippet: Immunoblot images were acquired with a UVP ChemStudio Imager (Analytik Jena Co., Upland, CA, USA) using Immobilon ® Western Chemiluminescent HRP Substrate (ECL; Merck KGaA, Darmstadt, Germany). .. The cochlear explants were fixed with 4% paraformaldehyde for 30 min at RT and then incubated with the permeabilization solution for 30 min. After the explants were washed, they were blocked with BlockPRO TM blocking buffer (Energenesis Biomedical Co., Ltd., Taipei, Taiwan). .. The cochlear explants were incubated with anti-myosin VIIa (Santa Cruz Biotechnology, Dallas, TX, USA), anti-4-HNE (Abcam, Cambridge, UK) and anti-iNOS (Novus Biologicals, LLC, Centennial, CO, USA) antibodies for 2 h at RT.

    Article Title: Notoginsenoside R1 Attenuates Cisplatin-Induced Ototoxicity by Inducing Heme Oxygenase-1 Expression and Suppressing Oxidative Stress
    Article Snippet: .. Aliquots of cell lysate were separated on mPAGE TM 4–12% Bis-Tris polyacrylamide gels (Merck KGaA, Darmstadt, Germany), transferred to polyvinylidene difluoride membranes (Millipore, Billerica, MA, USA), blocked with BlockPRO TM blocking buffer (Energenesis Biomedical Co., Ltd., Taipei, Taiwan), probed with anti-cleaved Caspase-3 (Cell Signaling Technology, Danvers, MA, USA), anti-HO-1 (Cell Signaling Technology, Danvers, MA, USA), and anti-actin (Merck KGaA, Darmstadt, Germany) antibodies at 4 °C overnight, washed with PBST, and incubated with horseradish peroxidase-conjugated secondary anti-rabbit or anti-mouse antibodies (1:10,000; Cytiva, Marlborough, MA, USA) diluted with BlockPRO TM blocking buffer (Energenesis Biomedical Co., Ltd., Taipei, Taiwan) for 1 h at room temperature (RT). .. Immunoblot images were acquired with a UVP ChemStudio Imager (Analytik Jena Co., Upland, CA, USA) using Immobilon ® Western Chemiluminescent HRP Substrate (ECL; Merck KGaA, Darmstadt, Germany).

    Staining:

    Article Title: Notoginsenoside R1 Attenuates Cisplatin-Induced Ototoxicity by Inducing Heme Oxygenase-1 Expression and Suppressing Oxidative Stress
    Article Snippet: .. The cells were incubated with polyclonal primary antibodies against 4-HNE (1:100; Abcam, Cambridge, UK) in BlockPRO TM blocking buffer (Energenesis Biomedical Co., Ltd., Taipei, Taiwan) in a humidified chamber for 1 h and then stained with a secondary antibody (donkey anti-rabbit conjugated to Alexa Fluor 488, 1:500; Molecular Probes; Thermo Fisher Scientific, Waltham, MA, USA) for an additional 1 h. Then, the cells were mounted in DAPI Fluoromount-G ® mounting medium. .. Images were captured with a confocal laser scanning microscope (Zeiss LSM 880, Carl Zeiss, Jena, Germany).



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